I think that IO::Scalar might be what you're looking for: 
use IO::Scalar;
my $data = <<EOF;
AAAAAAAAAAAAAAA
AAAAAAAGGAAACCA
EOF
my $SH = new IO::Scalar \$data;
my $in  = Bio::AlignIO->new(-fh => $SH, -format => 'fasta');

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In reply to Re: Converting Filehandle to Array call in Object Creation by davidrw
in thread Converting Filehandle to Array call in Object Creation by monkfan

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