This might also be a good place to use the extended (/x) regex syntax, with whitespace and comments, and strategic indenting, to better understand what's going on.
use strict;
use warnings;
"ctaacxxxxxxxxxxxxxxxxxxxxxxx" =~
/
( #begin $1
ct
(a|g) # $2
a
(c|t) # $3
) #end $1
(.{20,50})$ # $4
/ix;
print "\n$1,$2,$3,$4";
my ($branch,$pyrich)=($1,$4);
print "\n$branch$pyrich";
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