Use a non-greedy match:

#!/usr/bin/perl

use strict;
use warnings;

local $/=undef;
my $contents=<DATA>;
my ($thirdline) = $contents =~ m/Line3 : (.*?)\n/s;
print $thirdline."\n";


__DATA__
Line1 : Dit is de eerste regel
Line2 : Dit is de tweede regel
Line3 : Dit is de derde regel
Line4 : Dit is de vierde regel

[download]
Output: 
Dit is de derde regel

[download]
Or you could match against a negated character class: 
my ($thirdline) = $contents =~ m/Line3 : ([^\n]+)/s;

[download]

A computer is a state machine. Threads are for people who can't program state machines. -- Alan Cox

In reply to Re: Regular Expression tricky newline problem by tirwhan
in thread Regular Expression tricky newline problem by jkva

Title:
Use:  <p> text here (a paragraph) </p>
and:  <code> code here </code>
to format your post, it's "PerlMonks-approved HTML":


  • Posts are HTML formatted. Put <p> </p> tags around your paragraphs. Put <code> </code> tags around your code and data!
  • Titles consisting of a single word are discouraged, and in most cases are disallowed outright.
  • Read Where should I post X? if you're not absolutely sure you're posting in the right place.
  • Please read these before you post! —
  • Posts may use any of the Perl Monks Approved HTML tags:
    a, abbr, b, big, blockquote, br, caption, center, col, colgroup, dd, del, details, div, dl, dt, em, font, h1, h2, h3, h4, h5, h6, hr, i, ins, li, ol, p, pre, readmore, small, span, spoiler, strike, strong, sub, summary, sup, table, tbody, td, tfoot, th, thead, tr, tt, u, ul, wbr
  • You may need to use entities for some characters, as follows. (Exception: Within code tags, you can put the characters literally.)
            For:     Use:
    & &amp;
    < &lt;
    > &gt;
    [ &#91;
    ] &#93;
  • Link using PerlMonks shortcuts! What shortcuts can I use for linking?
  • See Writeup Formatting Tips and other pages linked from there for more info.