I think this meets the spec 

#! perl -slw
use strict;

my %pwm;
while( my $line = <DATA> ) {
    chomp $line;
    ++$pwm{ substr $line, $_, 1 }[ $_ ] for 0 .. length( $line ) -1;
}
my $n = $.;
@$_ = map{ $_ ? $_ / $n : 0 } @$_ for values %pwm;

print "$_ => @{ $pwm{ $_ } }" for keys %pwm;

__DATA__
AAA
ATG
TTT
GTC

[download]

Produces

C:\test>530623
A => 0.5 0.25 0.25
T => 0.25 0.75 0.25
C => 0 0 0.25
G => 0.25 0 0.25

[download]
Examine what is said, not who speaks -- Silence betokens consent -- Love the truth but pardon error.
Lingua non convalesco, consenesco et abolesco. -- Rule 1 has a caveat! -- Who broke the cabal?
"Science is about questioning the status quo. Questioning authority".
In the absence of evidence, opinion is indistinguishable from prejudice.

In reply to Re: Position Weight Matrix of Set of Strings by BrowserUk
in thread Position Weight Matrix of Set of Strings by monkfan

Title:
Use:  <p> text here (a paragraph) </p>
and:  <code> code here </code>
to format your post, it's "PerlMonks-approved HTML":


  • Posts are HTML formatted. Put <p> </p> tags around your paragraphs. Put <code> </code> tags around your code and data!
  • Titles consisting of a single word are discouraged, and in most cases are disallowed outright.
  • Read Where should I post X? if you're not absolutely sure you're posting in the right place.
  • Please read these before you post! —
  • Posts may use any of the Perl Monks Approved HTML tags:
    a, abbr, b, big, blockquote, br, caption, center, col, colgroup, dd, del, details, div, dl, dt, em, font, h1, h2, h3, h4, h5, h6, hr, i, ins, li, ol, p, pre, readmore, small, span, spoiler, strike, strong, sub, summary, sup, table, tbody, td, tfoot, th, thead, tr, tt, u, ul, wbr
  • You may need to use entities for some characters, as follows. (Exception: Within code tags, you can put the characters literally.)
            For:     Use:
    & &amp;
    < &lt;
    > &gt;
    [ &#91;
    ] &#93;
  • Link using PerlMonks shortcuts! What shortcuts can I use for linking?
  • See Writeup Formatting Tips and other pages linked from there for more info.