Well, if the file is not too big, a simple (but slightly convoluted) way to do that would be to read all the lines into an array, edit the first and last elements of the array, and then write the file back out again. Like so:
open IN, "<", "file.txt" or die "Cannot open file.txt for reading:$!\n
+";
my @lines = <IN>;
$lines[0] =~ s/,+$/,/;
$lines[-1] =~ s/,+$/,/;
open OUT, ">", "file.txt" or die "Cannot open file.txt for writing:$!\
+n";
print OUT for @lines;
close OUT;
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