Introduction

I am working with (physical) array data, that is scanned in at 100 microns / pixel. The result of this is a tif image with many spots corresponding to the points of interest on the array, which each have varied intensities. I have already figured out how to split these tif images into smaller files so that each point of interest is its own file. Now I am trying to figure out the next step - that is, I want to analyze each one independently. Each spot essentially has a "background" and a "data" region, that I could specify roughly by pixel locations.
I should also mention that there will be many of these arrays as time goes on, so I am looking of course for a reproducible method that I can repeat for any number of arrays as time goes on.

Question

Can I take these files into perl, and then do a comparison of the color (in this case, the greyscale value) of pixel (A,B) in the image versus (C,D) in the same image?

In reply to Perl Image Analysis by Anonymous Monk

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