To get you started, Tk::HList (Tk::Tree) colors are best controlled by Tk::ItemStyle. So it's really a question of setting up your styles, and browsecmd, etc, to do exactly what you want. Here is an example I had handy.

#!/usr/bin/perl
#------------------------
use strict;

use Tk;
use Tk::ItemStyle;
use Tk::Tree;

my $TK_top = MainWindow->new();

# create tree
my $TK_tree = $TK_top->Scrolled(
    'Tree',
    -background       => 'white',
    -selectbackground => 'black',
    -selectforeground => 'hotpink',
    -scrollbars       => 'osoe',
    -browsecmd        => \&TK_tree_FileSelect,
    -command          => \&TK_tree_FileSelect
  )->form(
    -left   => '%0',
    -top    => '%5',
    -bottom => '%70',
    -right  => '%95'
  );

# create style for different colors
my $Style_Red = $TK_tree->ItemStyle(
    'text',
    -foreground => 'red',
    -background => 'white',
    -selectforeground =>'purple',
);

my $Style_Blue = $TK_tree->ItemStyle(
    'text',
    -foreground => 'blue',
    -background => 'white',
    -selectforeground =>'orange',
);

my $Style_Green = $TK_tree->ItemStyle(
    'text',
    -stylename => 'green',
    -foreground => 'green',
    -background => 'white',
    -selectforeground =>'cyan',
);

my $Style_Black = $TK_tree->ItemStyle(
    'text',
    -foreground => 'black',
    -background => 'white',
    -selectforeground =>'yellow',
);

# create exit button
my $TK_Button_exit = $TK_top->Button( -font => 'code' )->form(
    -right  => [ '%100', '-20' ],
    -bottom => [ '%100', '-20' ]
);
$TK_Button_exit->configure(
    -text    => 'Exit',
    -relief  => 'raised',
    -width   => 30,
    -height  => 1,
    -command => sub { exit; }
);

PopulateTree();
MainLoop();

sub PopulateTree {
    my $entry;

    $entry = $TK_tree->addchild(
        "",
        -text     => "RED",
        -data     => "",
        -itemtype => 'text'
    );
    $TK_tree->entryconfigure( $entry, -style => $Style_Red );

    $entry = $TK_tree->addchild(
        "",
        -text     => "GREEN",
        -data     => "",
        -itemtype => 'text'
    );
    $TK_tree->entryconfigure( $entry, -style => $Style_Green );

    $entry = $TK_tree->addchild(
        "",
        -text     => "BLUE",
        -data     => "",
        -itemtype => 'text'
    );
    $TK_tree->entryconfigure( $entry, -style => $Style_Blue );

    $entry = $TK_tree->addchild(
        "",
        -text     => "BLACK",
        -data     => "",
        -itemtype => 'text'
    );
    $TK_tree->entryconfigure( $entry, -style => $Style_Black );

    $entry = $TK_tree->addchild(
        "",
        -text     => "RED",
        -data     => "",
        -itemtype => 'text'
    );
    $TK_tree->entryconfigure( $entry, -style => $Style_Red );

    $entry = $TK_tree->addchild(
        "",
        -text     => "GREEN",
        -data     => "",
        -itemtype => 'text'
    );
    $TK_tree->entryconfigure( $entry, -style => $Style_Green );
}

sub TK_tree_FileSelect {
    my $entry = shift;
    if (@_) {
        my $style = $TK_tree->itemCget( $entry, 0, -style );
        if ( $style eq $Style_Green ) {
            $TK_tree->configure( -selectbackground => 'gray45' );
        }
        elsif ( $style eq $Style_Red ) {
            $TK_tree->configure( -selectbackground => 'red' );
        }
        elsif ( $style eq $Style_Blue ) {
            $TK_tree->configure( -selectbackground => 'blue' );
        }
        elsif ( $style eq $Style_Black ) {
            $TK_tree->configure( -selectbackground => 'black' );
        }
        return;

         $TK_tree->update();
    }
}
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