It gets to be a real juggling act when you try to finesse the colors in these lists. You always run into a situation where one setting interferes with another. You just need to experiment. Try this:

#!/usr/bin/perl
use warnings;
use strict;
use Tk;
use Tk::Tree;
use Tk::ItemStyle;

my $fgcolor = "#000000";                   # foreground color
my $bgcolor = "#FFFFFF";                   # background color

my $text_font = 'helvetica 12';            # Body text font
my $mw = MainWindow->new();

$mw->optionAdd("*background", $bgcolor);
$mw->optionAdd("*foreground", $fgcolor);
$mw->optionAdd("*rotext*font", $text_font);
$mw->optionAdd("*tree*font", $text_font);
$mw->optionAdd("*entry*font", $text_font);

my $tree;
$tree = $mw->Scrolled('Tree', 
           -font=>$text_font, 
       -scrollbars=>'ose', 
       -selectmode=>'extended', 
       -selectbackground=>'white', 
       -highlightcolor=>'white',
       -highlightthickness => 0,
       -drawbranch=>0, 
       -browsecmd=>sub {
               foreach ($tree->selectionGet) {
                  if (/\./) {  # do not allow selection of top level i
+tems
               $tree->configure(-selectbackground => 'blue') 
         }else{
            #  $tree->selectionClear($_);
                      $tree->configure(-selectbackground => 'white')
            } 
        }
   $tree->anchorClear()
   }
)->pack(-side=>'top', -fill=>'both', -expand=>1);

$tree->columnWidth(0, -char, 90); #get full row color

# create style for different colors
my $S1 = $tree->ItemStyle(
    'imagetext',
    -foreground => 'black',
    -background => 'white',
    -selectforeground =>'black',
    -selectbackground =>'white',
);

my $S2 = $tree->ItemStyle(
    'imagetext',
    -foreground => 'black',
    -background => 'white',
    -selectforeground =>'yellow',
    -selectbackground =>'blue',
);

for my $top ( 1 .. 3) {
    $tree->add( $top, -text => $top, -style=> $S1 );
        
       for ( 'A' .. 'C') {
           $tree->add( "$top.$_", -text => "$_", -style=> $S2 );
        }
}

MainLoop();
[download]

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