Hi guys, I need help desperately. I have got to parse 8GB file containing FASTA format sequence files. How can I do it with PERL ?? I have to extract only the unique Fasta headers along with sequence. Suppose for ">ELKSMKO02JGD0L" I also need to get the alphabetical string of it TCAGGAATCTAATACTCAAGCT..... I will be obliged if Monks can help me out... The problem gets complex bcoz its a huge 8GB file. The file looks like this:- >ELKSMKO02JGD0L TCAGGAATCTAATACTCAAGCTGTGGCCTATCCAGTACAACATGTAGCGAGACAATAATATCTCAGGATC TGAATACACCCCTTCTGTTAAAATGCAGTCTAGGATTACACTAGCTTTGTTCACAGCCACGTAACACCAC TGACTCACATGAAGACTGAAGACAACACAACCCCCCACATCTTGTTCACAAAAACTGGTAGCATGCCAGG TCTTCCATATCTTTACAGGACACTTGGTATTTTACAAAACTTAATTC >ELKSMKO02FEYZW TCAGTCATAATGTCATTTCTTCAAAACTTGATCTGTAGATTTAATGGAACCCCAATCAAAATTCCAGCAA ATTATTAAGTGGATATCCACAAACTGGTTCAAAAGTTTATATGAAATAACAAAAGATCCAGAACAGCCAA CATAATATTGAAGGAGAAGAATGAAGTTCGAGGTCTAACAAACTAATTTCTGTATGATTCCAACTACATG GCATTCTGGAAAATGAAAAATTACAGACACAGTAAATAGCTCAGTGATTGCCAGGTAGG >ELKSMKO02IX3A4 TCAGTCCCAACGTGCTGGGAGGGCGTGAGCCACGGTGCCCAGCCTTTTTATTTTTTATTTTTATTTTTAA TCTGTCTTGATTTTGCTTCCTTCCTAAACAGTTTTGGCTTCGTGATCACGTAAACCAAGAGTCACAAACT GAAATGCCATCAAGGGGCCAAGCAGGTAACAAAATTCAAGTCATACAGGTTCAATGTCTTAGTCACCCCA GGCTACAACAGAATATCATAGACTGGGTANCCTAATAATACAGATCATTTTCNCATGGTTCTAGAGGAC

In reply to Need to Parse 8 GB File by ashnator

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