My first thought was to use a hash-of-hashes structure. If your data is as sparse as your sample input looks, this may save a little memory; otherwise, GrandFather's hash-of-arrays is more straight-forward: 
use strict;
use warnings;

my %counts;
my $max = 0;
while (<DATA>) {
    chomp;
    my $code = (split)[-1];
    my $i = 0;
    for my $c (split //, $code) {
        $counts{$c}{$i++}++;
    }
    $max = $i if $i > $max;
}
$max--;

for my $c (keys %counts) {
    print "$c ";
    for my $i (0 .. $max) {
        if (exists $counts{$c}{$i}) {
            print " $counts{$c}{$i}";
        }
        else {
            print ' 0';
        }
    }
    print "\n";    
}

__DATA__
fred  ATGTTGTAT
fred1 ATCTTATAT
fred2 ATCTTATAT

[download]

prints: 

A  3 0 0 0 0 2 0 3 0
T  0 3 0 3 3 0 3 0 3
C  0 0 2 0 0 0 0 0 0
G  0 0 1 0 0 1 0 0 0

[download]

In reply to Re: To count letters (%identity) in DNA alignment by toolic
in thread To count letters (%identity) in DNA alignment by sedm1000

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