It seems SearchIO failed to parse your file.

Furthermore, I've found several questions, in:
my $in = new Bio::SearchIO(-format => 'blast', -file => ">".$maid_dir."\\".$maid."aln_hu +.aln", -report_type => 'blastn');


1: Are you sure you are reading a BLAST file, while your file extension is "aln", which seems to be a multiple sequence alignment?

2: Are you sure you need "-report_type"? In the documentation, it says only if you're reading a blast2seq result, you need to use that

In reply to Re: I don't understand error message by llancet
in thread I don't understand error message by lomSpace

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