"Would have been better for you if you showed us what you came up with ... but anyway, this is one way to do it

use Bio::SeqIO my $in = Bio::SeqIO->new(-file => "test.fa" , '-format' => 'Fasta'); my $out = Bio::SeqIO->new(-file => ">out.fa" , '-format' => 'Fasta'); while ( my $seq = $in->next_seq() ) { $out->write_seq($seq) if($seq->length > 500); } "

Thank you very much for suggesting this script and Bioperl. I have downloaded Bioperl and tried to get the script work with my FASTA file but it returns:

"Can't call method "next_seq" on an undefined value at parse_sequence_length.pl line 6".

Any suggestions?

Thanks!

In reply to Re^2: Parse DNA fasta file by william.orsi
in thread Parse DNA fasta file by Anonymous Monk

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