I noticed 2 changes that should be made. 
while (<DATA>) {
    chomp;
    my ($organism, @vals) = (split /\t/)[2..5];
    $freq{ $organism }++;

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The line sfter the read needs to be chomped. And, the split should occur on tabs, not on whitespace. (When I ran the test program above, I split on whitespace instead of tabs because the sample data I copied into the program was separated by spaces, not tabs).

As long as $organism itself doesn't contain spaces you could split on whitespace. But I don't know enough about your data.

Chris

In reply to Re^2: Statistics via hash- NCBI BLAST Tab Delimited file by Cristoforo
in thread Statistics via hash- NCBI BLAST Tab Delimited file by Paragod28

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