A simple solution:
use strict;
my %chr_hash;
while(<DATA>)
{
chomp;
my ($chr, $start, $end, $gene) = split(/\t/, $_);
my $chr_key = $chr."_".$start."_".$end;
#print "$chr_key\n";
push( @{ $chr_hash{$chr_key} }, $gene );
}
foreach my $key (keys %chr_hash)
{
my ($c, $s, $e) = split(/\_/, $key );
print "$c\t$s\t$e\t";
for my $g ( @ {$chr_hash{$key} } )
{
print "$g,";
}
print "\n";
}
__DATA__
chr1 12345 34567 gene1
chr1 12345 34567 gene2
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