Hello, Monks!

I am trying to write a quality file and I am getting an error (as follows) and I'm not sure how to fix it. Along with the error report I have test data. Let me know if you have any suggestions! Thank you very much!!

# test data
37 37 37 37 37 37 37 37 37 37 37 40 40 40 40 40 40 40 40 40 40 40 40 4
+0 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 4
+0 40 40 40 40 39 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 3
+7 35 35 35 35 35 35 30 30 30 28 28 28 30 30 32 32 35 37 37 37 37 37 3
+7 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 3
+5 35 35 37 37 34 32 32 32 32 32 32 32 30 16 15 15 15 24 27 26 26 13 1
+3 13 23 23 13 13 13 17 21 21 21 21 21 19 21 16 18 17 19 18

# error report
--------------------- WARNING ---------------------
MSG: seq doesn't validate with [0-9A-Za-z\*\-\.=~\\/\?], mismatch is  
+, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+ , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , 
+, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+ , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , 
+, , , , , , , , , , , , , , , , , , 
---------------------------------------------------

------------- EXCEPTION: Bio::Root::Exception -------------
MSG: Attempting to set the sequence to [37 37 37 37 37 37 37 37 37 37 
+37 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 
+40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 37 37 37 37 
+37 37 37 37 37 37 37 37 37 37 37 37 37 37 35 35 35 35 35 35 30 30 30 
+28 28 28 30 30 32 32 35 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 
+37 37 37 37 37 37 37 37 37 37 37 37 37 35 35 35 37 37 34 32 32 32 32 
+32 32 32 30 16 15 15 15 24 27 26 26 13 13 13 23 23 13 13 13 17 21 21 
+21 21 21 19 21 16 18 17 19 18] which does not look healthy
STACK: Error::throw
STACK: Bio::Root::Root::throw /usr/lib/perl5/site_perl/5.8.8/Bio/Root/
+Root.pm:357
STACK: Bio::PrimarySeq::seq /usr/lib/perl5/site_perl/5.8.8/Bio/Primary
+Seq.pm:270
STACK: Bio::PrimarySeq::new /usr/lib/perl5/site_perl/5.8.8/Bio/Primary
+Seq.pm:221
STACK: Bio::LocatableSeq::new /usr/lib/perl5/site_perl/5.8.8/Bio/Locat
+ableSeq.pm:109
STACK: Bio::Seq::Meta::Array::new /usr/lib/perl5/site_perl/5.8.8/Bio/S
+eq/Meta/Array.pm:167
STACK: Bio::Seq::Quality::new /usr/lib/perl5/site_perl/5.8.8/Bio/Seq/Q
+uality.pm:191
STACK: tagfinder.pl:257

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In reply to .qual File Writing Complication by twaddlac

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