Thanks everyoene for your help. This is the finished code in case you're interested.
#!/usr/bin/perl
use strict;
use warnings;
my $infile = $ARGV[0];
unless (open (INFILE, "$infile")){
print STDERR "Can't open $infile $!\n";
die;
}
my ($header, $count , $match) = ('',0,0);
print "\n\n";
#This algorithm will work only when the sequence is on one line
while (<INFILE>) {
chomp;
if ($_ =~ />(.*)/) { #a header line
$header = $1;
$count++; #keep running total of seque
+nce number
}
else { #not a header
my $i = 0;
while ( $_ =~ /([VILMFWCA]{8,})/g) {
my $domain = $1;
my $len = length $doma
+in;
$len--;
if ($i == 0){
print "Hydroph
+obic strecth found in: $header\n";
$match++;
$i++;
}
print "$domain\n";
print "The match was a
+t potistion: ", pos() - $len, "\n";;
}
if ($i > 0){
print "\n\n";
}
}
}
close INFILE;
print "Hydrophobic region(s) found in $match sequences out of $count s
+equences\n";
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