Using pack will speed this up enormously. Besides that he wanted the numbers before the strings, so default to 999 instead of 0):
$ref = [
map { $_->[0] }
sort { $a->[1] cmp $b->[1] }
map { my $srn = lc $_->seq_region_name;
[ $_, pack "sA*", $srn =~ /^[0-9]+$/ ? $srn : 999, $srn ]
}
@$ref ];
Enjoy, Have FUN! H.Merijn
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