If no selection is made $lst->curselection is undefined. Adding:

    return if ! defined $lst->curselection;
[download]

as the first line of sample will avoid the problem. However I strongly recommend that you refactor your code to completely avoid global variables. Consider:

#!/usr/bin/perl -w
use warnings;
use strict;
use Tk;

main();

sub main {
    my @lst_files = (0 ... 10);
    my $file;

    my $mw    = new MainWindow;
    my $frm1  = $mw->Frame(-bg => 'LightSkyBlue1')->pack(-fill => 'x')
+;
    my $frm11 = $frm1->Frame(-bg => 'LightSkyBlue1')->pack(-fill => 'x
+');
    my $lst   = $frm11->Scrolled(
        'Listbox',
        -width      => 50,
        -height     => 5,
        -scrollbars => 'oe',
        -selectmode => 'single'
        )->pack(-side => 'bottom');
    $frm1->Button(
        -width   => 15,
        -text    => "fill",
        -command => sub {fill_boxes($lst, \@lst_files, @_)}
        )->pack(-side => 'top');
    $frm1->Button(
        -width   => 15,
        -text    => "print",
        -command => sub {sample($lst, @_)}
        )->pack(-side => 'top');
    MainLoop;
}


sub fill_boxes {
    my ($lst, $lst_files) = @_;

    $lst->delete('0.0', 'end');
    $lst->insert('end', @$lst_files);
}


sub sample {
    my ($lst) = @_;

    return if ! defined $lst->curselection;

    if ($lst->curselection ne '') {
        #Extract Application File Selection
        my $file = $lst->get($lst->curselection);
        print "$file\n";
    }
    # and then more happens
}
[download]

You can achieve similar results using OO techniques, but the important part is to ensure you pass the correct variables through to your callback routines.

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