You are actually not looping over the inner file, as on the 1st iteration you read to the end of the file and dont reset to the begining, try adding a seek, something like: 

#!/usr/bin/perl -w
use strict;
use warnings;
my $num_args = $#ARGV + 1;
if ($num_args != 3) {
  print "\nUsage: ./fastqkokku fasta quala outputfile\n";
  exit;
}
my $fasta=$ARGV[0];
my $quala=$ARGV[1];
my $out=$ARGV[2];
open  FA, "< $fasta"or die "Can't open $fasta: $!";
open  QA, "< $quala" or die "Can't open $quala: $!";
open  OUT,"> $out" or die "Can't open $out: $!";
     
while (my $line = <QA>){
    chomp $line;
    my $nextline = <QA>;
    while (my $compline = <FA>)
    {
        if ($compline =~ m/$line/)
        {
            print OUT "$compline$nextline"; 
        }
    }
    seek(FA,0,0);      
}
close FA;
close QA;
close OUT;

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In reply to Re: Compare two files, nested while loops? Outer loop not iterating? by monkey_boy
in thread Compare two files, nested while loops? Outer loop not iterating? by naturalsciences

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