Hi all, i have a question hoping you can answer me: I have a tk script with 2 Buttons and a Scrolled text windows: 1. Button ("OPEN") 2. Button ("Get Statistics") When i push the "Open" button i choose a .txt file and then the content is printed out in the Scrolled windows. Up to now no problem! The difficulty is this: When i push the "Get Statistic Button" it should call an external Script (that works fine alone) but i wish it worked on the opened file. (opened before with the "Open" button. Can you help me, please ? Here is the tk code and the script i would like to integrate into. The Tk script: 

#The Open button:

$mw-> Button (-text =>'Open',-command=>\open_file)->place(-x=>240, -y=
+>35); 


sub open_file {my $open = $mw->getOpenFile(
                               -filetypes => $types_OPEN,
                              -defaultextension => '.sff');
                                      $te->Load( "$open");}



#The Get Statistic button:

$mw-> Button (-text =>'Get Statistics', -command =>\&get_statistics); 

sub get_statistics {-------Here i need your help----};

# The Scrolled windows:

$te = $mw->Scrolled( q/TextUndo/, -scrollbars => 'se', -background....
+.......);

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The External (get_statistic.pl) Script (it works fine like: "perl get_statistic.pl filename.txt") 
# The get_statistic.pl script:

use Statistics::Descriptive;

my $stat = Statistics::Descriptive::Full->new();
my (%distrib);
my @bins = qw/18 19 20 21 22 23 24 25 26 27 28/;

my $fastaFile = shift;
open (FASTA, "<$fastaFile");
$/ = ">";

my $junkFirstOne = <FASTA>;

while (<FASTA>) {
    chomp;
    my ($def,@seqlines) = split /\n/, $_;
    my $seq = join '', @seqlines;
    $stat->add_data(length($seq));}


%distrib = $stat->frequency_distribution(\@bins);

print "Total reads:\t" . $stat->count() . "\n";
print "Total nt:\t" . $stat->sum() . "\n";
print "Mean length:\t" . $stat->mean() . "\n";
print "Median length:\t" . $stat->median() . "\n";
print "Mode length:\t" . $stat->mode() . "\n";
print "Max length:\t" . $stat->max() . "\n";
print "Min length:\t" . $stat->min() . "\n";
print "Length\t# Seqs\n";
foreach (sort {$a <=> $b} keys %distrib) {
    print "$_\t$distrib{$_}\n";
}

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Now i would like to run this script on the $open file and see the result in a dialog box windows. Please help !!! Thank you very much as always.

In reply to Perl tk - How to integrate external scripts by Giorgio C

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