Thank for your answer but unfortunately it still doesn't work. The code with your suggestions is: 
# the firs button :
 $mw-> Button (-text =>'Open', -command =>\&open_file)->place(-x=>240,
+ -y=>35); 



#The seocnd button:
$mw-> Button (-text =>'Get Statistics',-command =>\&get_statistics)->p
+lace(-x=>320, -y=>35); 

# The sub open :
sub open_file {my $open = $mw->getOpenFile(
                               -filetypes => $types_OPEN,
                              -defaultextension => '.sff');
                                      $te->Load( "$open");}
if (defined $open and -e $open){ 
        $te->Load( "$open");
        $current_file = $open;
}



# the sub get statistic:

sub get_statistics {my $stat = Statistics::Descriptive::Full->new();
my (%distrib);
my @bins = qw/18 19 20 21 22 23 24 25 26 27 28/;
my $fastaFile = shift;
   unless (defined $fastaFile){
        warn "No file name supplied.\n";
        return;
   }
   open my $FASTA, '<', $fastaFile or warn "Couldn't open file: $!\n";
my $junkFirstOne = <FASTA>;

while (<$FASTA>) {chomp;
    my ($def,@seqlines) = split /\n/, $_;
    my $seq = join '', @seqlines;
    $stat->add_data(length($seq));}


%distrib = $stat->frequency_distribution(\@bins);

print "Total reads:\t" . $stat->count() . "\n";
print "Total nt:\t" . $stat->sum() . "\n";
print "Mean length:\t" . $stat->mean() . "\n";
print "Median length:\t" . $stat->median() . "\n";
print "Mode length:\t" . $stat->mode() . "\n";
print "Max length:\t" . $stat->max() . "\n";
print "Min length:\t" . $stat->min() . "\n";
print "Length\t# Seqs\n";
foreach (sort {$a <=> $b} keys %distrib) {
    print "$_\t$distrib{$_}\n";
}};

# The scrolled window:
$te = $mw->Scrolled( q/TextUndo/, -scrollbars => 'se', -background => 
+'white', -font => [-family =>'arial',-size => '12',-weight=> 'bold'],
+-wrap => 'none',)->pack(-anchor =>'n',-side=>'right',-fill=>'none');

[download]
It say me (after load the $open file in the scrolled window and push the get_statistic button) : "No file name supplied". Where i need to correct the script to make it works ? Thanks

In reply to Re^4: Perl tk - How to integrate external scripts by Giorgio C
in thread Perl tk - How to integrate external scripts by Giorgio C

Title:
Use:  <p> text here (a paragraph) </p>
and:  <code> code here </code>
to format your post, it's "PerlMonks-approved HTML":


  • Posts are HTML formatted. Put <p> </p> tags around your paragraphs. Put <code> </code> tags around your code and data!
  • Titles consisting of a single word are discouraged, and in most cases are disallowed outright.
  • Read Where should I post X? if you're not absolutely sure you're posting in the right place.
  • Please read these before you post! —
  • Posts may use any of the Perl Monks Approved HTML tags:
    a, abbr, b, big, blockquote, br, caption, center, col, colgroup, dd, del, details, div, dl, dt, em, font, h1, h2, h3, h4, h5, h6, hr, i, ins, li, ol, p, pre, readmore, small, span, spoiler, strike, strong, sub, summary, sup, table, tbody, td, tfoot, th, thead, tr, tt, u, ul, wbr
  • You may need to use entities for some characters, as follows. (Exception: Within code tags, you can put the characters literally.)
            For:     Use:
    & &amp;
    < &lt;
    > &gt;
    [ &#91;
    ] &#93;
  • Link using PerlMonks shortcuts! What shortcuts can I use for linking?
  • See Writeup Formatting Tips and other pages linked from there for more info.