There is something that you are not telling us. The only instance of "unallowed parameter" in the source code of Bio::Tools::Primer3 is

 $self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};

[download]

So, why don't you tell us which parameter it does not like? And why do you try to pass a wrong parameter at all? My guess is that the error also has a line number or other stack trace. Why do you keep it secret from us?

In reply to Re: Primer3 - BioPerl by Corion
in thread Primer3 - BioPerl by blarsen20

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