Does it matter either way?

Of course it matters, but that's application specific.

What zwon says, and then:

• If your subroutines running time is so long that the overhead of setting up the database connection is negligible, you would delegate setting up the handle to each subroutine.
• If your subs comprise transactions and you need to close the connection after each, you would let the sub set up the connection.
• If you have many small operations, scattered over subroutines each running for a short time, you would pass the handle into the subs.
• If database stuff is merely an initializing task, or a side aspect of your application, you might want to encapsulate the database stuff into a subroutine.
• In a single threaded architecture, you could use one handle. In a multithreaded environment, you'd create a handle in subroutines which comprise a thread.

Then you might have long running tasks. Could there be time-outs? Does your application fork? and so on...

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