Yes, the original mistake was mine. I have since updated that node. I don't know any slick way of putting it all in one character class, though with lookahead it could be wedged into one regex.

Update: here's my first shot at a lookahead version:

my @strings = qw(
    aaaaa
    eeeee
    iiiii
    abcde
    aeiou
    bdfhj
);

my $range = 'AaEeIiOoUu';
my $alt   = join '|', split //, $range;

my %regexes = (
    alternation => qr/$alt/,
    class       => qr/[$range]/,
    lookahead   => qr/([$range])((?!\1)[$range])((?!\2)[$range])((?!\3
+)[$range])((?!\4)[$range])/,
);

foreach my $string (@strings)
{
    print "$string:\n";
    while (my ($k, $r) = each %regexes)
    {
        print "\t$k - " . ($string =~ /$r/ ? 'YES' : 'NO') . $/;
    }
}
[download]

And the output:

aaaaa:
        alternation - YES
        class - YES
        lookahead - NO
eeeee:
        alternation - YES
        class - YES
        lookahead - NO
iiiii:
        alternation - YES
        class - YES
        lookahead - NO
abcde:
        alternation - YES
        class - YES
        lookahead - NO
aeiou:
        alternation - YES
        class - YES
        lookahead - YES
bdfhj:
        alternation - NO
        class - NO
        lookahead - NO
[download]

if ($somestring =~ /^(?=.*?u)(?=.*?i)(?=.*?o)(?=.*?a)(?=.*?e)/i) {
  print "$somestring contains all five vowels.\n";
}
[download]

Checking in order of least to most frequent in English, of course.

-- Randal L. Schwartz, Perl hacker
Be sure to read my standard disclaimer if this is a reply.