Not yet, I'll be setting that up this afternoon.
You beat me to the punch on that one. I was already planning it. :D
Update: Here is the original sub's run times:
Total Elapsed Time = 1135.701 Seconds
User+System Time = 770.4718 Seconds
Exclusive Times
%Time ExclSec CumulS #Calls sec/call Csec/c Name
63.8 491.8 491.86 1 491.86 491.86 main::WEED
32.5 251.1 251.10 22012 0.0114 0.0114 main::SEARCHFASTA
And here it is without the switch to grep, but keeping the sorting fixes you suggested, as well as most of your other helps:
Total Elapsed Time = 789.7615 Seconds
User+System Time = 741.4615 Seconds
Exclusive Times
%Time ExclSec CumulS #Calls sec/call Csec/c Name
65.4 485.3 485.35 1 485.35 485.35 main::WEED
33.5 248.8 248.86 22012 0.0113 0.0113 main::SEARCHFASTA
0.96 7.120 7.120 1 7.1200 7.1200 main::INDEX_FASTA
So, removing the double sorting shaved off 6.5 seconds. It's a start.. Now, to try and shave off another 300 or so more....
Matt
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