You'll get an error if one of these variables contains a single quote '.

To avoid that (and to boost performance) use placeholders instead:

my $sth = $dbh->prepare("INSERT INTO ques(username, userque, ...) VALU
+ES (?, ?, ...)");
foreach my $t (split '~', $txtque){
     $sth->execute($usernamenow, $t, ...);
}
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