Tk::Error: Can't call method "rooty" without a package or object

Yes, because there is no Frame subwidget to the Pane. The following does the trick:

# Configure the Scrollbar to scroll each Listbox
$scroll->configure(
    -command => sub {
                  my $delta = shift;
                  foreach my $list (@$listboxes) {
                      $list->yview('scroll', $delta, 'units');
                }
    }
);
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See the yview method in Tk::Pane.