Like this

#!/usr/local/bin/perl -w

# adapted from
# http://search.cpan.org/src/LUSOL/Tk-Splashscreen-1.0/splashscreen.pl

package Tk::Splashinfo;
use Tk::widgets qw/Toplevel/;
use base qw/Tk::Toplevel/;

Construct Tk::Widget 'Splashinfo';

sub Populate {
    my ( $self, $args ) = @_;
    $self->withdraw;
    $self->overrideredirect(1);
    $self->SUPER::Populate($args);
}

1;

package main;
use Tk;
use Tk::Splashscreen;
use Tk::widgets qw/Photo Animation/;
use strict;

my $mw = MainWindow->new;
$mw->Button( -text => 'Quit', -command => \&exit )->pack;

{
    my $gif89   = Tk->findINC('anim.gif');
    my $splash  = $mw->Splashinfo();
    my $animate = $splash->Animation( -format => 'gif', -file => $gif8
+9 );
    $splash->Label( -image => $animate )->pack;
    $animate->set_image(0);
    $animate->start_animation(500);

    $splash->Popup;    # show Splashscreen

    $mw->Button(
        -text    => 'Kill splash',
        -command => sub { $splash->destroy }
    )->pack;

}

$mw->deiconify;        # show calculator

MainLoop;
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