Quick and dirty:

use strict;
use warnings;

while (<DATA>) {
    my $time = (split /,/)[1];
    my ($hr) = $time =~ /^"(\d\d)/;
    print if $hr>=5 and $hr<=23;
}

__DATA__
"02-feb-2010","04:59:19;06 ","00:00:15;00 "   ,"creeid00402           
+  ","OY00402W","OY00402W","610D/00","04:00:00;00 ","04:00:14;29.","02
+-feb-2010","04:59:34;06 "
"02-feb-2010","04:59:34;06 ","00:29:01;25 "   ,"ihw-14ubc-q           
+  ","3316291-","3316291-","610E/00","03:00:00;00 ","03:29:01;24.","02
+-feb-2010","05:28:35;29 "
"02-feb-2010","05:28:35;29 ","00:00:15;00 "   ,"creeid00402           
+  ","OY00402W","OY00402W","610D/00","04:00:00;00 ","04:00:14;29.","02
+-feb-2010","05:28:50;29 "
"02-feb-2010","05:28:50;29 ","00:00:30;00 "   ,"scifim10078           
+  ","SF10078W","SF10078W","610F/00","02:00:00;00 ","02:00:29;29.","02
+-feb-2010","05:29:21;01 "
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If any of your fields could have commas, you'd be better off with a CSV parser, such as Text::CSV_XS.