Another alternative is to use Inline::C. This reads 'Myfile' correctly for me:

use warnings;
use Inline C => <<'EOC';

#define DSZ 108

struct stud
{
        char name[100];
        int roll_no;
};

struct student
{
        struct stud s;
        int grade;
}value;

void foo(char * fn) {
     FILE *fps;
     int n;
     struct student *buffer=(struct student*)malloc(sizeof(struct stud
+ent));
     fps=fopen(fn,"rb");
     n=fread(buffer,DSZ,1,fps);
     printf("Name:%s Grade:%d NO:%d\n",buffer->s.name,buffer->grade,bu
+ffer->s.roll_no);
     fclose(fps);
}

EOC

foo("Myfile");
# outputs:
# Name:myname Grade:1 NO:149
[download]

It's trivial to return the values to perl, if need be (rather than simply print them to stdout). And if it's necessary to use perl's IO abstraction interface (see perldoc perlapio) instead of the usual C functions, then I think that could be accommodated, too.

Cheers,
Rob