You try this,

use warnings;
open FILE, "Testus.txt" or die "Couldn't open file.";
while (<FILE>) { print "$_ \n" if(/^(\d+\.\d+\t(\d+)\.\d+)/ and grep {
+$2} (41 .. 249)};
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First match will check for digit,followed by period,then digit,then tab and finally digit in a line and second grep will check the third digit in the match is greater than 40 and less than 250.