in reply to how to construct multiple sequence alignment from group of pairwise alignments

Have you looked at http://www.bioperl.org? This is not a script writing service and we don't really know much about DNA either. So, if you have a specific Perl problem, show your code, part of your input data, the output you get and the output you want.

If you want advice on the general approach, I think you will have to change your problem description to require less biologic background.

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