Hello, I tell you that mplayer if I work very well in my distro, I even work on GTK2-perl but only when the container is of the window, vbox, hbox. The problem is when I want to use inside a container type LAYOUT and the hierarchy of the code is something like this:

* With these examples work but I see no video, only audio.
# Window-> vbox-> Layout-> MPlayerEmbed (not working)
# Window-> Layout-> vbox-> MPlayerEmbed (not working)

* This example works but does not meet my purpose.
# Window-> vbox-> MPlayerEmbed (works but does not help in this way)

The code I've left, works to a degree that is stuck. This is what I have in your sample code:

#!/usr/bin/perl -w
use warnings;
use strict;
use Glib qw/TRUE FALSE/;
use Gtk2;
use Gtk2 '-init';
use Proc::Killfam;

my $pid;  #global for killing mplayer on exit
my $window = Gtk2::Window->new('toplevel');
$window->set_title('Embed test');
$window ->signal_connect( 'destroy' => \&delete_event );
$window->set_border_width(10);
$window->set_size_request(600,600);


my $vbox = Gtk2::VBox->new( FALSE, 6 );
$window->add($vbox);
$vbox->set_border_width(2);

my $hbox= Gtk2::HBox->new( FALSE, 6 );
$vbox->pack_end($hbox,FALSE,FALSE,0);
$hbox->set_border_width(2);


$vbox->pack_end (Gtk2::HSeparator->new, FALSE, FALSE, 0);

my $vbox1 = Gtk2::VBox->new( FALSE, 6 );
$vbox1->set_size_request(600,500);
$vbox->pack_end($vbox1,FALSE,FALSE,0);
$vbox1->set_border_width(2);

my $button = Gtk2::Button->new_from_stock('gtk-quit');
$hbox->pack_end( $button, FALSE, FALSE, 0 );
$button->signal_connect( clicked => \&delete_event );

my $button1 = Gtk2::Button->new('Mplayer');
$hbox->pack_end( $button1, FALSE, FALSE, 0 );
$button1->signal_connect( clicked => \&do_mplayer );

$window->show_all();
Gtk2->main;
#####################################
sub delete_event {
    killfam 9, $pid;
    Gtk2->main_quit;
    return FALSE;
}  

##########################################
sub do_mplayer{

    my $gdkwindow = $window->window;
    print "gdkwin->$gdkwindow\n";    

    my $gdkwindow1 = $vbox1->window;
    print "gdkvbox->$gdkwindow1\n";    
    my $xid = $gdkwindow1->XWINDOW;

    print "xid->$xid\n";

    my $url ='';

    my @options = ( '-slave','-loop 0', '-x 600', '-y 450', '-really-q
+uiet', '-wid $xid' );

    my $mpg = 'my_video.mp4';
    my $init = 'my_video.mp4';
    $pid = open(MP, "| mplayer @options $init "); 
    syswrite(MP, "loadfile $mpg\n");
}
########################################
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The script runs fine but not up mplayer.