I need to keep the seperators as it is

A bit tedious then:

#!/usr/bin/perl
use warnings;
use strict;

use Test::More;

sub mask {
    my $num = shift;
    my ($begin, $end) = (q{}, q{});;
    $begin .= substr $num, 0, 1, q{} until       7 == $begin =~ tr/0-9
+//;
    $num = substr($begin, -1, 1, q{}) . $num;
    $end = substr($num, -1, 1, q{}) . $end until 5 == $end   =~ tr/0-9
+//;
    $num .= substr $end, 0, 1, q{};
    $num =~ tr/0-9/X/;
    return $begin . $num . $end;
}

is(mask($_->[0]), $_->[1]) for (
    ['1234 5678 1234 5678'    , '1234 56XX XXXX 5678'],
    ['1234567812345678'       , '123456XXXXXX5678'],
    ['5413 21069873210200'    , '5413 21XXXXXXXX0200'],
    ['541321069873210200'     , '541321XXXXXXXX0200'],
    ['1234/1234-5678-9012:34' , '1234/12XX-XXXX-XX12:34']
);

done_testing();
[download]

Update: Fixed error in tr, thanks, jwkrahn.

    $begin .= substr $num, 0, 1, q{} until       7 == $begin =~ tr/[0-
+9]//;
...
    $end = substr($num, -1, 1, q{}) . $end until 5 == $end   =~ tr/[0-
+9//;
...
    $num =~ tr/[0-9]/X/;
[download]

You are including the [ and ] characters but the OP only wants to change numerical digits.

    $begin .= substr $num, 0, 1, q{} until       7 == $begin =~ tr/0-9
+//;
...
    $end = substr($num, -1, 1, q{}) . $end until 5 == $end   =~ tr/0-9
+//;
...
    $num =~ tr/0-9/X/;
[download]