in reply to Another Reverse Complement Question...
#!/usr/bin/perl use strict; use warnings; use 5.014; use Bio::PrimarySeq; my $seqobj = Bio::PrimarySeq->new(-seq => 'ACTGTGGCGTCAACTG', -alphabet => 'dna', -id => 'test'); say $seqobj->seq; say $seqobj->revcom->seq; __END__ *** print out ACTGTGGCGTCAACTG CAGTTGACGCCACAGT
But this doesn't do the translation you want: A => T, C => G, G => C and T => A. So, I don't know if this is any help.
Update: After examining the reversed complement sequence, I see indeed it performs the complement as you stated but it also reverses the sequence with the first letter in the original being complemented and last in the reversed sequence, the second in the original complemented and being next to last in the reversed complement sequence, etc.
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