----------------------------------------------
 Welcome to Strawberry Perl Portable Edition!
 * URL - http://www.strawberryperl.com/
 * see README.portable.TXT for more info
----------------------------------------------
Perl executable: C:\_64\strawberry516\perl\bin\perl.exe
Perl version   : 5.16.0

C:\_64\strawberry516>cd \_64\perl1400\bin

C:\_64\perl1400\bin>gendef perl514.dll
 * [perl514.dll] Found PE+ image

C:\_64\perl1400\bin>dlltool --kill-at --input-def perl514.def --output
+-lib libperl514.a

C:\_64\perl1400\bin>
[download]

Hey Rob, Thanks to ur instructions this time I have generated the libperl514.a of 860KB size. :) But now during the make test message is prompted that "The program can't start because libgcc_s_sjlj-1.dll is missing from your computer. Try reinstalling the program to fix this problem." Output is here

  CPAN.pm: Going to build K/KA/KARASIK/Win32-GuiTest-1.60.tar.gz

Set up gcc environment - gcc.exe (rubenvb-4.5.4) 4.5.4
Checking if your kit is complete...
Looks good
Writing Makefile for Win32::GuiTest
Writing MYMETA.yml
cp lib/Win32/GuiTest/GuiTest.pc blib\lib\Win32\GuiTest\GuiTest.pc
cp lib/Win32/GuiTest/Examples.pm blib\lib\Win32\GuiTest\Examples.pm
cp lib/Win32/GuiTest.pm blib\lib\Win32\GuiTest.pm
cp lib/Win32/GuiTest/Cmd.pm blib\lib\Win32\GuiTest\Cmd.pm
C:\Perl64\bin\perl.exe -IC:\Perl64\lib -IC:\Perl64\lib C:\Perl64\lib\E
+xtUtils\xsubpp  -typemap C:\Perl64\lib\ExtUtils\typemap -typemap perl
+object.map -typemap
gcc -c          -O2 -DWIN32 -DPERL_IMPLICIT_CONTEXT -DPERL_MSVCRT_READ
+FIX -O2     -DVERSION=\"1.60\"    -DXS_VERSION=\"1.60\" -DHASATTRIBUT
+E "-IC:\Perl64\lib\C
gcc -c          -O2 -DWIN32 -DPERL_IMPLICIT_CONTEXT -DPERL_MSVCRT_READ
+FIX -O2     -DVERSION=\"1.60\"    -DXS_VERSION=\"1.60\" -DHASATTRIBUT
+E "-IC:\Perl64\lib\C
Running Mkbootstrap for Win32::GuiTest ()
C:\Perl64\bin\perl.exe -MExtUtils::Command -e "chmod" -- 644 GuiTest.b
+s
C:\Perl64\bin\perl.exe -MExtUtils::Mksymlists \
     -e "Mksymlists('NAME'=>\"Win32::GuiTest\", 'DLBASE' => 'GuiTest',
+ 'DL_FUNCS' => {  }, 'FUNCLIST' => [], 'IMPORTS' => {  }, 'DL_VARS' =
+> []);"
dlltool --def GuiTest.def --output-exp dll.exp
g++ -o blib\arch\auto\Win32\GuiTest\GuiTest.dll -Wl,--base-file -Wl,dl
+l.base -s -mdll -L"C:\Perl64\lib\CORE" GuiTest.o DibSect.o   C:\Perl6
+4\lib\CORE\libperl51
dlltool --def GuiTest.def --base-file dll.base --output-exp dll.exp
g++ -o blib\arch\auto\Win32\GuiTest\GuiTest.dll -s -mdll -L"C:\Perl64\
+lib\CORE" GuiTest.o DibSect.o   C:\Perl64\lib\CORE\libperl514.a -lmsv
+crt -lmoldname -lker
C:\Perl64\bin\perl.exe -MExtUtils::Command -e "chmod" -- 755 blib\arch
+\auto\Win32\GuiTest\GuiTest.dll
C:\Perl64\bin\perl.exe -MExtUtils::Command -e "cp" -- GuiTest.bs blib\
+arch\auto\Win32\GuiTest\GuiTest.bs
C:\Perl64\bin\perl.exe -MExtUtils::Command -e "chmod" -- 644 blib\arch
+\auto\Win32\GuiTest\GuiTest.bs
  KARASIK/Win32-GuiTest-1.60.tar.gz
  dmake -- OK
Running make test
C:\Perl64\bin\perl.exe "-MExtUtils::Command::MM" "-e" "test_harness(0,
+ 'blib\lib', 'blib\arch')" t/*.t
t/01_basic.t ..... Can't load 'C:\Perl64\cpan\build\Win32-GuiTest-1.60
+-5fvYWK\blib\arch/auto/Win32/GuiTest/GuiTest.dll' for module Win32::G
+uiTest: load_file:Th
 at t/01_basic.t line 12
Compilation failed in require at t/01_basic.t line 12.
BEGIN failed--compilation aborted at t/01_basic.t line 12.
t/01_basic.t ..... Dubious, test returned 2 (wstat 512, 0x200)
No subtests run
t/02_calc.t ...... skipped: this test is phased out
t/03_cpl.t ....... Can't load 'C:\Perl64\cpan\build\Win32-GuiTest-1.60
+-5fvYWK\blib\arch/auto/Win32/GuiTest/GuiTest.dll' for module Win32::G
+uiTest: load_file:Th
 at t/03_cpl.t line 19
Compilation failed in require at t/03_cpl.t line 19.
BEGIN failed--compilation aborted at t/03_cpl.t line 22.
t/03_cpl.t ....... Dubious, test returned 2 (wstat 512, 0x200)
No subtests run
t/04_std.t ....... Can't load 'C:\Perl64\cpan\build\Win32-GuiTest-1.60
+-5fvYWK\blib\arch/auto/Win32/GuiTest/GuiTest.dll' for module Win32::G
+uiTest: load_file:Th
 at t/04_std.t line 12
Compilation failed in require at t/04_std.t line 12.
BEGIN failed--compilation aborted at t/04_std.t line 15.
t/04_std.t ....... Dubious, test returned 2 (wstat 512, 0x200)
No subtests run
t/05_which.t ..... ok
t/06_sendkeys.t .. Can't load 'C:\Perl64\cpan\build\Win32-GuiTest-1.60
+-5fvYWK\blib\arch/auto/Win32/GuiTest/GuiTest.dll' for module Win32::G
+uiTest: load_file:Th
 at t/06_sendkeys.t line 12
Compilation failed in require at t/06_sendkeys.t line 12.
BEGIN failed--compilation aborted at t/06_sendkeys.t line 12.
t/06_sendkeys.t .. Dubious, test returned 2 (wstat 512, 0x200)
No subtests run

Test Summary Report
-------------------
t/01_basic.t   (Wstat: 512 Tests: 0 Failed: 0)
  Non-zero exit status: 2
  Parse errors: No plan found in TAP output
t/03_cpl.t     (Wstat: 512 Tests: 0 Failed: 0)
  Non-zero exit status: 2
  Parse errors: No plan found in TAP output
t/04_std.t     (Wstat: 512 Tests: 0 Failed: 0)
  Non-zero exit status: 2
  Parse errors: No plan found in TAP output
t/06_sendkeys.t (Wstat: 512 Tests: 0 Failed: 0)
  Non-zero exit status: 2
  Parse errors: No plan found in TAP output
Files=6, Tests=6, 425 wallclock secs ( 0.03 usr +  0.03 sys =  0.06 CP
+U)
Result: FAIL
Failed 4/6 test programs. 0/6 subtests failed.
dmake.exe:  Error code 130, while making 'test_dynamic'
  KARASIK/Win32-GuiTest-1.60.tar.gz
  dmake test -- NOT OK
//hint// to see the cpan-testers results for installing this module, t
+ry:
  reports KARASIK/Win32-GuiTest-1.60.tar.gz
Running make install
  make test had returned bad status, won't install without force
Failed during this command:
 KARASIK/Win32-GuiTest-1.60.tar.gz            : make_test NO
[download]

This dll file is available in strawberry516. So, can I copy this dll file from strwaberry perl C:_\strawberry516\c\bin to C:\Perl64\bin and again rerun the installation and check.

...program can't start because libgcc_s_sjlj-1.dll is missing

I haven't used any of Ruben's builds of MinGW, but I would have expected that there would be a libgcc_s_sjlj-1.dll in the same ('bin') folder as gcc.
Search the entire compiler tree for that file and, if you find it, add that location to your PATH.
I think you might find it in both the compiler's mingw\lib and x86_64-w64-mingw32\lib folder - in which case you just add either of those locations to the path.

This dll file is available in strawberry516

Best not to copy it across to a different compiler.
You could use the compiler that came with Strawberry instead of Ruben's, if you like. Just put the strawberry/c/bin folder first in the path.

However, Ruben's should be fine, I think ... once you find libgcc_s_sjlj-1.dll and add it's location to the path.

Cheers,
Rob

Hi Rob, Thanks once again for your help. I have copied all dll files present in strawberry perl to c:\perl64\bin and ran the installation. Thankfully, I was able to install Win32::GuiTest module on perl64 successfully. But my problem still not solved reason being, I have a automated framework suite where I have used Win32::GuiTest module to test the GUI functionality of the windows application 32 bit and 64 bit. This module perfectly working fine for 32 bit application but for 64 bit application it fails for APIs like GetPopupHandle, GetListContents etc. Moreover with this I have one more module named "TreeView.pm" (Win32::GuiTest::TreeView) this module basically traverse the systreeview class and provide us the relevant information but this module also not working properly on windows 64 bit application. So, unfortunately again I got stuck and wondering now what to do and also want to know that this module Win32::GuiTest compiled as 64 bit by 64 bit compiler or its simply installed as 32 bit. Thanks.