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  1. or download this
     use strict;
use Getopt::Long; 
use lib qw(/opt/scripts/Modules/);
...
            "p|prefixe=s"=>\$prefixe,
            "mc|min_read_classe=s"=>\$seuil_min_classe,
            "a|max_read_per_cent_ambi=s"=>\$seuil_max_amb);
  2. or download this
     use strict;
use Getopt::Long; 
use lib qw(/opt/scripts/Modules/);
...
            "a|max_read_per_cent_ambi=s"=>\$seuil_max_amb||1);#default
+ value : 1