Dear Masters,
I have the following data and problem:
my $k =25;
my %readrepo =(
"readA" => "GCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCA",
"readB" => "TACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAAC",
"readC" => "GCTGAGGCAGGAGAATTGCTTGAACTTAGGGGATG",
"readD" => "TACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAAC",
);
# This array is already ordered
# It says the first(_1) 25 bases of readA overlap with second(_2)part
+of readB, etc.
my @readstoconcate = (
"readA_1",
"readB_2",
"readC_1",
"readD_2");
What I want to do is to assemble the reads in
%readrepo based on the order
given by
@readstoconcatenate
readA GCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCA
readB TACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAAC
readC GCTGAGGCAGGAGAATTGCTTGAACTTAGGGGATG
readD TACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAAC
Yielding the final long stretch of sequence
TACTC(A,G)GGAGGAGAATTGCTTGAACCTGGGAGGCA(T,C)T(A,G)GG(A,G)G(A,G)(T,C)(A
+,G)
How should one go about it?
Update: Corrected the answer bug above. Thanks to BrowserUK suggestion.
---
neversaint and everlastingly indebted.......
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