Dear Masters,
I have the following data and problem:
my $k =25; my %readrepo =( "readA" => "GCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCA", "readB" => "TACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAAC", "readC" => "GCTGAGGCAGGAGAATTGCTTGAACTTAGGGGATG", "readD" => "TACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAAC", ); # This array is already ordered # It says the first(_1) 25 bases of readA overlap with second(_2)part +of readB, etc. my @readstoconcate = ( "readA_1", "readB_2", "readC_1", "readD_2");
What I want to do is to assemble the reads in %readrepo based on the order given by @readstoconcatenate
readA GCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCA readB TACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAAC readC GCTGAGGCAGGAGAATTGCTTGAACTTAGGGGATG readD TACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAAC
Yielding the final long stretch of sequence
TACTC(A,G)GGAGGAGAATTGCTTGAACCTGGGAGGCA(T,C)T(A,G)GG(A,G)G(A,G)(T,C)(A +,G)
How should one go about it?
Update: Corrected the answer bug above. Thanks to BrowserUK suggestion.

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neversaint and everlastingly indebted.......

In reply to Mustering Reads by neversaint

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