Will it fit your needs to just strip all the whitespace from the end of the string?

open FILE, '<', 'test.file' or die;
while(<FILE>){
  s/\s+$//;
  print "line=[$_]\n";
}
close FILE;
[download]

Note that you don't want to use chomp because you're looking to remove more than just $/ ... and you don't want to use chop because you don't want to accidentally remove a piece of the folder name..