hello perlbird and welcome to the monastery!
I do not know what reshape2 has to offer, but your immediate request would be a fairly simple data manipulation exercise. Along with examples in previous reply.
#!usr/bin/perl
use warnings;
use strict;
my %genetable;
while(<DATA>){
chomp;
next if /^#/;
my ($genekey, $samplevalue ) = split /\t/;
push @{ $genetable{$genekey} }, $samplevalue;
}
print map { "$_ @{$genetable{$_}}\n" } sort keys %genetable;
exit 0;
__END__
#omitted the header row
gene_A sample_1
gene_B sample_1
gene_C sample_1
gene_B sample_2
gene_C sample_2
gene_A sample_3
--------
gene_A sample_1 sample_3
gene_B sample_1 sample_2
gene_C sample_1 sample_2
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