I am writing a perl program to find the longest open reading frames in a mRNA.fa sequence. I can easily do this by calling the file from the script using:

open FILE, "mRNA.fa" or die $!; # Opens mRNA, assoicates FILE

However, my instructor wants the file to be called using command line prompt

perl <programName.pl> <input file>

Now I have tried using the command ARGV to pull the filename from the bash:

my $file = $ARGV[0]; open (my $mRNAfile, "<", $file) or die $!; my @mRNAdata = ( ); # initalizes the variables of array @mRNAdata = <$mRNAfile>;

When I run this, I receive compilation errors for each of the 100 sequences saying:

"Number found where operator expected at - line 1, near "Sequence 1" ( +Do you need to predeclare Sequence?).

Any advice would be greatly appreciated! Thank you!


In reply to Shell calling input FASTA file by JoUthoff

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