In addition to what others have said, these two lines look like a problem:
my $newRefFile = "$pathName\newRefFile.txt";
my $newBlastFile = "$pathName\newBlastFile.txt";
Those backslashes won't show up the way you expect unless you double them -- all they're doing here is inserting a newline in the strings. If $pathName is being defined the same way, the problem is compounded. I'm doubtful that this code works from the command line.
You might as well just use a plain old forward slash for separating directory names, because perl will know what you mean by that and do the Right Thing. (Or you could use the appropriate File:: module(s) to construct path names in an OS-neutral manner.)
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