What do you mean it "isn't working"? Are you sure your data really is what you think it is? Note that you don't need to \ the " inside the regexp, though it doesn't hurt. Here's test code that works for me. 
#!/usr/bin/perl -lw

use strict;
use warnings;

while(<DATA>){
  chomp;
  my @data = split /\t/;
  my $ddrul = $data[7];
  $ddrul =~ s/\"//g;
  print "'$ddrul'";
}

__DATA__
EST-NY    234    5-Oct    Springfield    MA    Springfield College    
+Townhouse Conference Room    http://www.spfldcol.edu/home.nsf/welcome
+/visit/directionsc
EST-NY    923    18-Oct    Salisbury    MD    Wor Wic Community Colleg
+e        http://www.worwic.edu/campus/directions.pdf
EST-NY    886    19-Oct    Frederick    MD    Hood College        http
+://www.hood.edu/welcome_to_hood/index.cfm?pid=_maps.htm#a1
SW    328    19-Oct    Houston    TX    University of Houston - Clear 
+Lake        "http://prtl.uhcl.edu/portal/page?_pageid=328,217631,328_
+217645&_dad=portal&_schema=PORTALP"

[download]
Prints: 
'http://www.spfldcol.edu/home.nsf/welcome/visit/directionsc'
'http://www.worwic.edu/campus/directions.pdf'
'http://www.hood.edu/welcome_to_hood/index.cfm?pid=_maps.htm#a1'
'http://prtl.uhcl.edu/portal/page?_pageid=328,217631,328_217645&_dad=p
+ortal&_schema=PORTALP'

[download]

In reply to Re: Replacing a pesky pair of quotes. by Eimi Metamorphoumai
in thread Replacing a pesky pair of quotes. by hmbscully

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