use Win32::ODBC;
$DSN = "DRIVER=Microsoft Access Driver (*.mdb);FIL=MSAccess;DriverId=25;DBQ=F:/webroot/Database/db1.mdb";
                                                          (this is the actual location of your .mdb file) 

##</code><code>##

$db=new Win32::ODBC($DSN);	
and run a query:
$db->Sql("SELECT * FROM Table");
$db->FetchRow();
%hash = $db->DataHash;
$db->Close();