Can't call method "next_hit" on an undefined value at read_blast_stats.pl line
    150, <GEN2> line 253 (#1)
    (F) You used the syntax of a method call, but the slot filled by the
    object reference or package name contains an undefined value.  Something
    like this will reproduce the error:
    
        $BADREF = undef;
        process $BADREF 1,2,3;
        $BADREF->process(1,2,3);
    
Uncaught exception from user code:
    Can't call method "next_hit" on an undefined value at read_blast_stats.pl line 150, <GEN2> line 253.
 at read_blast_stats.pl line 150

##</code><code>##

# Creates BioPerl input sequence stream.
my $in_seq = Bio::SeqIO->new(-file   => "<$input_file", -format => $seq_format );

# Extracting sequences from stream.
print("- reading and translating sequences...\n");
my $seq_counter = 0;
my $unique_count = 0;
my @translated_seqs;

while (my $seq = $in_seq->next_seq()) 
{
    
    $seq_counter++;
    print("-\tsequences read: $seq_counter\r");
    
    my $temp_seq;
    
    # Translates sequence and stores the translated sequences if they do not
    # contain any stop codons.
    
    # Reading frames 1, 2 and 3.
    $temp_seq = $seq->translate();
    $temp_seq->display_id("test_id-$unique_count");
    $unique_count++;
    push(@translated_seqs, $temp_seq) unless ($temp_seq->seq() =~ /\*+/);
    $temp_seq = $seq->translate(-frame => 1);
    $temp_seq->display_id("test_id-$unique_count");
    $unique_count++;
    push(@translated_seqs, $temp_seq) unless ($temp_seq->seq() =~ /\*+/);
    $temp_seq = $seq->translate(-frame => 2);
    $temp_seq->display_id("test_id-$unique_count");
    $unique_count++;
    push(@translated_seqs, $temp_seq) unless ($temp_seq->seq() =~ /\*+/);

    # Creates the reverse complement sequence.
    my $rc_seq = $seq->revcom();
    
    # Reading frames -1, -2, and -3
    $temp_seq = $rc_seq->translate();
    $temp_seq->display_id("test_id-$unique_count");
    $unique_count++;
    push(@translated_seqs, $temp_seq) unless ($temp_seq->seq() =~ /\*+/);
    $temp_seq = $rc_seq->translate(-frame => 1);
    $temp_seq->display_id("test_id-$unique_count");
    $unique_count++;
    push(@translated_seqs, $temp_seq) unless ($temp_seq->seq() =~ /\*+/);
    $temp_seq = $rc_seq->translate(-frame => 2);
    $temp_seq->display_id("test_id-$unique_count");
    $unique_count++;
    push(@translated_seqs, $temp_seq) unless ($temp_seq->seq() =~ /\*+/);
    
}

# Blasting...
my @params = (-program => 'blastp', -database => '/share/raid1/database/ftp.ncbi.nih.gov/blast/db/20090602/nr', -expectation => '200000', -Word => '2', -Matrix => 'PAM30', -Gapcost => '9.1', -FilterString => 'F', _READMETHOD => 'blasttable' , -m => '8', -outfile => 'blast.out');
my $blaster = Bio::Tools::Run::StandAloneBlast->new(@params);
my $blast_report = $blaster->blastall(@translated_seqs);

# Parsing results
my $result = $blast_report->next_result();
my $hit = $result->next_hit();

##</code><code>##

# This code is inserted instead of 
# the Blasting and parsing part of the original code.

# Creating blast.in
open(OUTPUT_FILE, "> blast.in") or die "Could not create the output file \"blast.in\"\n";

# Writing blast.in sequence file in FASTA format
foreach(@translated_seqs){
    print OUTPUT_FILE (">".$_->id()."\n".$_->seq()."\n");}

# Performing blast
`blastall -i "blast_beta.in" -d /share/raid1/database/ftp.ncbi.nih.gov/blast/db/20090602/nr -p blastp -e 200000 -W 2 -M PAM30 -G 9.1 -F F -m 8 -o "blast.out"`;

# Creating SearchIO
my $blast_report = Bio::SearchIO->new(-format => "blasttable", -file  => "blast.out");

# Testing SearchIO for data.
my $result = $blast_report->next_result();
my $hit = $result->next_hit();
print("Number of hits: ".$hit->length()."\n");