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  1. or download this
    CTACTCTCTCGTTTCCTAGGCTC
-1.06
CTACTCTCTCGTTTCCTAGGCTC
...
-17.26
TCGCCAAGGTCAGTGGCACAACT
-31.06
  2. or download this
    CTACTCTCTCATTTCCTAGGCTC
-1.82
CTACTCTCTCATTTCCTAGGCTC
...
-17.87
TCGCCAAGGTCAGTAGCACAACT
-35.97
  3. or download this
    [whatever sequence is]
[difference between 2 values].
  4. or download this
    #!/usr/bin/perl -w
use strict;

...

use Data::Dumper;
print Dumper @calc;
  5. or download this
    #!/usr/bin/perl -w
use strict;

...
print "The difference is:", "\n";
use Data::Dumper;
print Dumper @calc;