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++$index{ $1 } while $$rSeq =~ m[(?=([ACGT]{7}))]g;

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[ 6:55:26.00] C:\test\humanGenome>..\976237 chr21.fa
16384
...
Using custom index2 found 35106546 matches; took 27.504099 seconds
Using custom index3 found 35106546 matches; took 27.571143 seconds
Using quantified charclass lookahead found 35106546 matches; took 49.9
+54810 seconds