Dear Monks,
I am trying to use LWP::Simple to get sequences from a website. The web address for each sequence is always the same except for the sequence id at the end. I am only retrieving 537/603 sequences with the below code.
Obviously it seems that some of my sequences can't be found. However, if it can't find a sequence I am trying to print an error message to say so in the output - this is the part that i can't get to work.
I wondered if someone could suggest how I can print an error message if the sequence can't be found (e.g. web site is empty or doesn't exist). Thanks!
use strict; use LWP::Simple; my @fasta; my $o; # Note: @accession defined earlier has 603 elements for (my $i=0; $i<@accession; $i++) { if ($accession[$i] =~ /^(\w+)\_(\w+)\_(\w+)\;/) { #print "$accession[$i]\n"; if ($1 eq $3) { # USE $1 AS THE ACCESSION NUMBER my $seq = get "http://us.expasy.org/cgi-bin/get-sprot- +fasta?$1"; #print "$seq\n"; push (@fasta, $seq); push @fasta, "> COULDN'T FIND IT" unless defin +ed $seq; } else { # # USE $3 AS THE ACCESSION NUMBER my $seq = "http://us.expasy.org/cgi-bin/get-sprot-fas +ta?$3"; push (@fasta, $seq); push @fasta, "> COULDN'T FIND IT" unless defin +ed $seq; } } } #print "$o\n"; # fasta only has 537 / 603 elements print "@fasta\n";

In reply to LWP::Simple problems by Anonymous Monk

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