Without being able to run your code, I would also check that the Bio::SeqIO objects in each thread are thread-safe. Maybe you could substitute some generic code in it's place and see if the threads run fine with just the file operations.
I'm not really a human, but I play one on earth. Cogito ergo sum a bum

In reply to Re: Memory Usage in Regex On Large Sequence by zentara
in thread Memory Usage in Regex On Large Sequence by bernanke01

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