Hi, I've been getting protein sequences from the NCBI for years using code like this: 
  
use LWP;
use HTTP::Request::Common;

my $ua = new LWP::UserAgent;
    
my $result = $ua->request(GET 
"http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&qty=1&start
+=1&list_uids=148261691&dopt=fasta");

if($result->is_success){
    $fasta = $result->content;
}

# then parse $fasta to get out just my protein sequence

[download]
However, this no longer works as the NCBI have started using javascript. If you copy in the url above and view the page source you'll see what I mean - the sequence is no longer visible in the page source. The sequence should start

>gi|148261691|ref|YP_001235818.1| CheA signal transduction histidine kinase Acidiphilium cryptum JF-5 MTGGGSMDPMAEIRETFFQECEEQLAELESGLMRMEAGETDSETVNAVFRAVHSIKGGAGAFGLEDLVHF

Can anyone tell me how to get my sequences now? Thanks, Becky

In reply to Getting data from NCBI by Becky

Title:
Use:  <p> text here (a paragraph) </p>
and:  <code> code here </code>
to format your post, it's "PerlMonks-approved HTML":


  • Posts are HTML formatted. Put <p> </p> tags around your paragraphs. Put <code> </code> tags around your code and data!
  • Titles consisting of a single word are discouraged, and in most cases are disallowed outright.
  • Read Where should I post X? if you're not absolutely sure you're posting in the right place.
  • Please read these before you post! —
  • Posts may use any of the Perl Monks Approved HTML tags:
    a, abbr, b, big, blockquote, br, caption, center, col, colgroup, dd, del, details, div, dl, dt, em, font, h1, h2, h3, h4, h5, h6, hr, i, ins, li, ol, p, pre, readmore, small, span, spoiler, strike, strong, sub, summary, sup, table, tbody, td, tfoot, th, thead, tr, tt, u, ul, wbr
  • You may need to use entities for some characters, as follows. (Exception: Within code tags, you can put the characters literally.)
            For:     Use:
    & &amp;
    < &lt;
    > &gt;
    [ &#91;
    ] &#93;
  • Link using PerlMonks shortcuts! What shortcuts can I use for linking?
  • See Writeup Formatting Tips and other pages linked from there for more info.